In the 1930s, in Northeast China, in a luxury hotel called "Peace Hotel", a group of mysterious guests were locked in. They formed alliances in a closed space and launched a life-and-death game for ten days and 240 hours, which affected the world situation at that time. Wang Dading, the second-in-command of the Heixiaziling bandits in Aodong City, Northeast China, accidentally entered the Peace Hotel while tracking down a batch of opium paste that was about to flow into the people, and met Chen Jiaying, a behavioral trace analysis expert specially hired by the South Railway Intelligence Department. At this moment, Sheriff Dou Shixiao ordered the blockade of the entire Peace Hotel in order to track down the editor who sneaked into the Peace Hotel with important evidence films. As a latent Communist Party member, Chen Jiaying had to pretend to be a couple with Wang Dading, who met by chance, to avoid the investigation of the Japanese and puppet enemies in order to send the film containing evidence of Japan's testing of bacterial weapons in Northeast China out of the Peace Hotel and break through the siege of the Japanese gendarmerie. At the same time, as a microcosm of the world's political landscape, every guest staying at the Peace Hotel is not simple. Representatives from Japan, Germany, the United States, France, the Soviet Union, the Central Bureau of Investigation and the Military Bureau of Investigation will cooperate and struggle in the Peace Hotel.